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KMID : 0363320010220020135
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Journal of Korean Oriental Internal Medicine
2001 Volume.22 No. 2 p.135 ~ p.144
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The Effects of Woohwangcheongsim-won on Hypoxic E18 Cortical Neuroblast
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Cho Gyu-Seon
Jeong Seung-Hyun
Shin Gil-Cho
Moon Il-Soo
Lee Won-Chul
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Abstract
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Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast.
Methods : To evaluate the effect of Woohwangcheongsim-won on neuronal death caused by hypoxia, the survival rate of E18 cortical neuroblast was measured with MTT assay and the changes of several synaptic proteins and enzymes were investigated with the immunoblot assays.
Results : The E18 cortical neuroblasts were added 50, 100, 500, 1,000, and 5,000{mu}g/ml Woohwangcheongsim-won. They showed neurotoxicity, when the concentration of Woohwangcheongsim-won was above 1,000{mu}g/ml. The E18 cortical neuroblasts, which were added 50, 100, and 500{mu}g/ml Woohwangcheongsim-won, were exposed 98% N_2/5%;CO_2 for 3 hours to induce hypoxia, 3 days later, the survival rate of 50{mu}g/ml Woohwangcheongsim-won was 141.5% when compared to the control group. On the immuneblot assays, the expressions of {alpha}CaMKII, NR2A, NR28, PDE2, PSD-95, and eEF-1{alpha} were increased in normoxia, but those of NR2A, NR2B were decreased in hypoxia when compared to the control group.
Conclusions : The data shows that the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast is a significant result.
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KEYWORD
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Woohwangcheongsim-won, hypoxic cortical neuroblast, immunoblot
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